Super-resolution imaging of PD

The recent development of super-resolution imaging techniques in cell biology opens up enormous opportunities in plant biology. The small size of PD (about 40nm diameter) is below the limit of resolution of conventional fluorescence-based imaging techniques and makes then ideal candidates for imaging with photoactivation localisation microscopy (PALM). In this project, we are collaborating with Cristina Flors, School of Chemistry, University of Edinburgh http://www.chem.ed.ac.uk/staff/academic/flors.html who has constructed a PALM imaging system within the Centre for Collaborative Optical Spectroscopy, Micromanipulation and Imaging (COSMIC https://www.wiki.ed.ac.uk/display/Cosmic/COSMIC+Home). As proteomics approaches identify new proteins that are associated with the PD pore, PALM opens up enormous opportunities for localising PD-associated proteins at super-resolution.